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Morphological diversity and practical maturation of human astrocytes in glia-enriched cortical organoid transplanted in mouse brain

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Data accessibility

snRNA-seq information are readily available in Gene Expression Omnibus (GEO) under the accession GSE185472. The following public datasets were utilized for snRNA-seq analysis: Allen Brain Institute human adult snRNA-seq information from several cortical locations (https://portal.brain-map.org/atlases-and-data/rnaseq/human-multiple-cortical-areas-smart-seq; accessed October 2022), snRNA-seq information from broad temporal protection from fetal to their adult years phases of the Brodmann location 8, 9, 10 and 46 prefrontal cortex areas (GEO accession GSE168408) and snRNA-seq from 8-month-old cortical organoid transplants (GEO accession GSE190815). For single-nucleus analysis, we utilized hg19 human recommendation genome v1.2.0 and mm10 mouse referral genome v1.2.0 supplied by 10x Genomics. The series and gene files utilized to construct the recommendations can be accomplished at ftp:// ftp.ensembl.org/pub/grch37/release-84/fasta/homo_sapiens/dna/ and ftp:// ftp.ensembl.org/pub/grch37/release-84/gtf/homo_sapiens/ (for human hg19 genome); ftp:// ftp.ensembl.org/pub/release-84/fasta/mus_musculus/dna/ and ftp:// ftp.ensembl.org/pub/release-84/gtf/mus_musculus/ (for mouse mm10 genome). All other raw information utilized for outlining in the figures are supplied as source information. Source information are offered with this paper.

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